Birthcohort | Birthcohorts

Cohort, name
The Uppsala-Stockholm Assisted Reproductive Techniques Study

Cohort, abbreviation
UppStART

Cohort, country
Sweden (Sverige)

Cohort, website
https://ki.se/en/meb/uppstart

Key reference
Iliadou AN, Öberg AS, Pege J, et al The Uppsala–Stockholm Assisted Reproductive Techniques (UppStART) study. BMJ Open 2019;9:e028866. doi: 10.1136/bmjopen-2018-028866

Date of filling in this questionnaire:
2019-09-05
Principal investigator(s), name
Carolyn Cesta, Sara Öberg

Principal investigator(s), e-mail address
carolyn.cesta@ki.se, sara.oberg@ki.se

Principal investigator(s), institution
Karolinska Institute

Contact person(s), name
Carolyn Cesta

Contact person(s), e-mail address
carolyn.cesta@ki.se

Contact person(s), institution address
Karolinska Institutet

Main aim of cohort
The Uppsala–Stockholm Assisted Reproductive Techniques (UppStART) study is a prospectively recruited sample of couples undergoing assisted reproduction in Stockholm and Uppsala county in Sweden. The study was initiated to (1) investigate possible changes in the epigenetic profile of infants inferred through the ART procedures and their consequence and (2) to assess the impact of lifestyle and health exposures on treatment outcome.

Recruited number of:
0 children / 514 mothers / 457 fathers / 0 grand parents / 0 other family members

Approximate proportion of source population included
0

Source population
Region-based
Period of enrollment
Start: 2011-09-01, End: 2013-12-31

Enrollment period
Pre pregnancy to

Enrollment criteria, please specify
-Couples planning their first IVF/ICSI treatment -an understanding of the Swedish language

Exclusion criteria, please specify
- use of donor gametes

Planned age of children at end of follow-up
Life-long / unrestricted

Questionnaire or registry data and biological samples

General information	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Home address
School address

Birth outcomes
Birth weight
Birth length
Gestational age at birth
Apgar score
Congenital malformations
Stillbirth (>= 22 weeks)
Child’s sex
Head circumference
Biparietal diameter

Child exposures	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Breast feeding
Diet
Dietary supplements
Childcare attendance
Active smoking
Passive smoking
Alcohol consumption
Physical activity
Substance use
Medicine intake
Vaccinations
Psychological distress (e.g. bullying)
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Child development and health	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Anthropometry
Weight
Height
Head circumference
Abdominal circumference
Arm circumference
Wrist circumference
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
Cardiac ultrasound
Renal ultrasound
Brain MRI
Other MRI
DEXA‐scan results
Skin folds
Bio impedence
Oscilometry
Spirometry
Skin prick test
Bronchial challenge test
Reversibility test
Dental observations
Mental and neurological development
Cognitive function
School performance
Language
ADHD
Autism
Sleep disturbances
Mental health
Respiratory health
Asthma
Allergy
Allergic rhinitis
Eczema
Respiratory tract infections
Senses
Vision
Hearing
Musculo-skeletal health
Back pain
Neck pain
Growing pains
Sexual maturation
Tanner stage
Pubertal development stage
Age at menarche
Age at voice change
2D/4D digit ratio
Nocturnal ejaculation
Acne
Other health outcomes
Cancer
Diabetes
Congenital anomalies
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Child biological samples	---------- Child age (years) at sampling ----------
	At birth	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Teeth
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Umbilical cord blood/tissue	X
Blood smear
Faeces/meconium
Exhaled breathe condensate
Sputum induction
Placenta	X

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education	X
Income	X
Employment status	X
Job title	X
Single parenthood	X
Home address
Work address

Maternal characteristics related to the index pregnancy/child
Age at birth	X
Ethnicity	X
Country of birth	X
Planned pregnancy	X
Time to pregnancy	X
Fertility treatment	X
Prior spontaneous abortion (<22w)	X
Prior births	X
Ultrasound measurements	X
Doppler measurements	X
Preeclampsia	X
Gestational diabetes	X
Gestational hypertension	X
Gestational duration	X
Mode of delivery	X
Prenatal diagnostics	X
Medical termination of pregnancy	X

Maternal Exposures	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Diet	X
Dietary supplements	X
Active smoking	X
Passive smoking	X
Alcohol consumption	X
Physical activity	X
Substance use	X
Medicine intake	X
Coffee drinking	X
Psychological distress (e.g. life-events, health behaviors, perceived stress)	X
Occupational exposures	X
Heavy lifts	X
Work hours	X
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Maternal health	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Anthropometry
Weight	X
Height	X
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bioimpedence
Mental and neurological development
Mental health	X
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma	X
Allergy	X
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer	X
Diabetes	X
Cardio‐vascular disease	X
Family history of chronic disease
Infectious disease	X
Fever
Autoimmune disease	X
Urinary symptoms
Sexual problems
Sleep disturbance	X
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol	X
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Maternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Breast milk
Whole blood	X
Serum	X
Plasma	X
Buffy coat
Urine
Hair
Nails
Saliva	X
Purified DNA	X
Purified RNA
Red blood cells
Faeces

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education	X
Income	X
Employment status	X
Job title	X
Single parenthood	X
Home address
Work address

Paternal characteristics related to the index pregnancy/child
Fertility treatment	X
Age at child's birth	X
Ethnicity	X
Country of birth	X

Paternal exposures	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Diet	X
Dietary supplements	X
Active smoking	X
Passive smoking	X
Alcohol consumption	X
Physical activity	X
Substance use	X
Medicine intake	X
Coffee drinking	X
Psychological distress (e.g. life-events, health behaviors, perceived stress)	X
Occupational exposures	X
Heavy lifts	X
Work hours	X
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Paternal health	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Anthropometry
Weight	X
Height	X
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bio impedence
Mental and neurological development
Mental health	X
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma	X
Allergy	X
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer	X
Diabetes	X
Cardio‐vascular disease	X
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease	X
Urinary symptoms
Sexual problems
Sleep disturbance	X
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol	X
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Paternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Semen
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces

Comments and ideas

Comments and ideas
Self-reported demographic, health and lifestyle data were collected from a baseline questionnaire, and to assess changes to lifestyle, a followup questionnaire was issued at the time of oocyte retrieval, and at subsequent IVF cycles. Questionnaire data were linked to data extracted from medical records. Biological samples were collected at baseline: blood for extraction of serum, plasma and DNA, morning and evening saliva samples for cortisol measurement and at delivery including samples of maternal blood, placenta and amniotic fluid, and cord blood for epigenetic analysis. Future plans: Through the unique identification number assigned to each Swedish citizen at birth or immigration, UppStART study participants will be linked to the Swedish population-based national and quality registers to provide data from prenatal, obstetrical, neonatal and infant care, and subsequent updates will provide data on childhood health and educational outcomes. Collaboration and use of UppStART data is encouraged, and more information about access can be found at www.ki.se/meb/uppstart