Identification

• Cohort, name
  Trieste child development cohort
  
  Cohort, abbreviation
  Trieste cohort
  
  Cohort, country
  Italy (Italia)
  
  Date of filling in this questionnaire:
  2012-03-15
  
  
• Principal investigator(s), name
  Luca Ronfani, Fabio Barbone
  
  Principal investigator(s), e-mail address
  ronfani@burlo.trieste.it
  
  Principal investigator(s), institution
  Institute for Maternal and Child Health IRCCS Burlo Garofolo, Trieste - Italy and Università degli Studi di Udine, Italy
  
  Contact person(s), name
  Luca Ronfani
  
  Contact person(s), e-mail address
  ronfani@burlo.trieste.it
  
  Contact person(s), institution address
  Institute for Maternal and Child Health IRCCS Burlo Garofolo, Trieste - Italy
  
  

Basic description

• Main aim of cohort
  The aim of the study is evaluate the relation between environmental, social, and genetic factors on neurocognitive development of children (cognitive and social skills, motor, language). In particular this study assesses the interaction between the effects of mercury exposure trough foods (specially fish) and other nutritional and toxic environmental factors on the developing nervous system, social indicators and the impact of gene-environment interations on infants. The primary hypotheses is that prenatal and/or postnatal MeHg exposure at low levels is associated with decreased neurobehavioral and neurocognitive function. A secondary objective is to assess the confounding effect of other nutritional factors (e.g. selenium, zinc, vitamins and essential fatty acids), environmental pollutants (e.g. lead, PCB’s, organochlorines), and various social factors on the health effects from MeHg. The Trieste Cohort includes the women enrolled in the PHIME study, Northen Adriatic Cohort II (NAc II), estending the follow up to 7 years
  
  Recruited number of:
  900 children / 900 mothers / 0 fathers / 0 grand parents / 0 other family members
  
  Approximate proportion of source population included
  2
  
  Source population
  Other, please describeCity-based
  
  
• Period of enrollment
  Start: 2007-01-01, End: 2009-01-01
  
  Enrollment period
  Pregnancy, week 20 to 22
  
  Enrollment criteria, please specify
  All women >=18 years of age at the time of the morphologic sonogram between 20 and 22 weeks gestational age
  
  Exclusion criteria, please specify
  - Women with chronic illnesses that may have a neurodevelopmental impact upon the unborn child (e.g. neurological diseases, seizure disorders, thyroid disease, etc.) - serious pregnancy complications (e.g. intrauterine growth retardation, preterm birth) - Drug abuse - Twin gestation - Language barriers - Residence in the region <2 years - Hair lenght insufficient for sample
  
  Planned age of children at end of follow-up
  7 years

Questionnaire or registry data and biological samples

• Children
• Mothers
• Fathers

General information	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Home address
School address

Birth outcomes
Birth weight	X
Birth length	X
Gestational age at birth	X
Apgar score
Congenital malformations	X
Stillbirth (>= 22 weeks)
Child’s sex	X
Head circumference
Biparietal diameter

Child exposures	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Breast feeding	X
Diet	X
Dietary supplements
Childcare attendance
Active smoking
Passive smoking	X
Alcohol consumption
Physical activity
Substance use
Medicine intake
Vaccinations
Psychological distress (e.g. bullying)
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Child development and health	---------- Child age (years) at assessment ----------
	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Anthropometry
Weight
Height
Head circumference
Abdominal circumference
Arm circumference
Wrist circumference
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
Cardiac ultrasound
Renal ultrasound
Brain MRI
Other MRI
DEXA‐scan results
Skin folds
Bio impedence
Oscilometry
Spirometry
Skin prick test
Bronchial challenge test
Reversibility test
Dental observations
Mental and neurological development
Cognitive function		X		X				X
School performance
Language		X		X				X
ADHD
Autism
Sleep disturbances
Mental health		X	X					X
Respiratory health
Asthma
Allergy
Allergic rhinitis
Eczema
Respiratory tract infections
Senses
Vision
Hearing
Musculo-skeletal health
Back pain
Neck pain
Growing pains
Sexual maturation
Tanner stage
Pubertal development stage
Age at menarche
Age at voice change
2D/4D digit ratio
Nocturnal ejaculation
Acne
Other health outcomes
Cancer
Diabetes
Congenital anomalies
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Child biological samples	---------- Child age (years) at sampling ----------
	At birth	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	>18
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair		X
Teeth
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Umbilical cord blood/tissue	X
Blood smear
Faeces/meconium
Exhaled breathe condensate
Sputum induction
Placenta

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address

Maternal characteristics related to the index pregnancy/child
Age at birth	X
Ethnicity	X
Country of birth
Planned pregnancy
Time to pregnancy
Fertility treatment
Prior spontaneous abortion (<22w)
Prior births	X
Ultrasound measurements
Doppler measurements
Preeclampsia
Gestational diabetes
Gestational hypertension
Gestational duration	X
Mode of delivery	X
Prenatal diagnostics
Medical termination of pregnancy

Maternal Exposures	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Diet					X
Dietary supplements					X
Active smoking					X
Passive smoking					X
Alcohol consumption					X
Physical activity
Substance use
Medicine intake					X
Coffee drinking					X
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposures					X
Heavy lifts
Work hours
Air pollution					X
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination					X
Binge drinking					X
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Maternal health	---------- Timing of assessment ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Anthropometry
Weight					X
Height					X
Waist circumference
Clinical examinations
Blood pressure					X
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bioimpedence
Mental and neurological development
Mental health			X		X
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal disease					X
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer					X
Diabetes					X
Cardio‐vascular disease					X
Family history of chronic disease
Infectious disease					X
Fever					X
Autoimmune disease					X
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Maternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	First trimester	Second trimester	Third trimester	Post pregnancy
Breast milk					X
Whole blood
Serum			X		X
Plasma
Buffy coat
Urine					X
Hair			X
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces

General information
	Pre-pregnancy	During pregnancy	<1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18
Education
Income
Employment status
Job title
Single parenthood
Home address
Work address

Paternal characteristics related to the index pregnancy/child
Fertility treatment
Age at child's birth	X
Ethnicity	X
Country of birth

Paternal exposures	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Diet
Dietary supplements
Active smoking			X
Passive smoking
Alcohol consumption
Physical activity
Substance use
Medicine intake
Coffee drinking
Psychological distress (e.g. life-events, health behaviors, perceived stress)
Occupational exposures			X
Heavy lifts
Work hours
Air pollution
Access to green spaces
Built environment
Noise
Radiation, electromagnetic fields
Water contamination
Binge drinking
Pets
Farm animal exposure
Biomarkers of exposure
Mercury
Lead
Cadmium
Arsenic
Other metals
Polychlorinated biphenyl esters
Dicloro difenil tricloroetano
Hexachlorobenzene
Brominated flame retardants
Perflouralkyl compounds
Bisphenol A
Other bisphenols
Parabens
Benzophenones
Triclosan
Phthalates
Cotinine

Paternal health	---------- Timing of assessment ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Anthropometry
Weight			X
Height			X
Waist circumference
Clinical examinations
Blood pressure
Carotida intima media thickness
Pulse wave velocity
DEXA scans
Bio impedence
Mental and neurological development
Mental health
Cognitive function (e.g. intelligence quotient)
Respiratory health
Asthma
Allergy
Musculo‐skeletal disease
Back pain
Neck pain
Arthritis
Other health outcomes
Cancer
Diabetes
Cardio‐vascular disease
Family history of chronic disease
Infectious disease
Fever
Autoimmune disease
Urinary symptoms
Sexual problems
Sleep disturbance
Biomarkers of health
Nephrology (e.g. creatinine, albumin)
Thyroid hormones
Inflammatory markers (e.g. C-reactive protein cytokines)
Immunologic markers (e.g. immunoglobulines)
Cortisol
Vitamines and micronutrients
Gonadal axis hormones (e.g. testosterone, estradiol)
Metabolism (e.g. cholesterol, LDL, HDL, insulin, triglycerides)
Metabolomics
DNA methylation
Transcriptomics (mRNA, miRNA)
Genome-wide association study (GWAS)

Paternal biological samples	---------- Timing of sampling ----------
	Pre-pregnancy	During pregnancy	Post pregnancy
Semen
Whole blood
Serum
Plasma
Buffy coat
Urine
Hair
Nails
Saliva
Purified DNA
Purified RNA
Red blood cells
Faeces

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